The expression pattern for srf-2 was explored using a promoter construct driving dsRedII; transgenic animals carrying this construct exhibited strong fluorescence in the seam cells.
Expressed in PVT, phasmids, AIA cells, mu_sph, M1, M5, and I5. The lacZ construct, but not a GFP construct (see Expression pattern 539), was expressed in AWC cells.
Expressed in PVT, phasmids, AIA cells, mu_sph, M1, M5, and I5. A lacZ construct, but not this GFP construct (see Expression pattern 538), was expressed in AWC cells.
The pPHA2::GFP-D construct, which contains the third intron as well as part of the fourth exon, exhibited an expression profile similar to the pPHA2::GFP-E construct (Expr11836).
The pfn-3 promoter::GFP fusion construct yielded no expression, suggesting that an additional enhancer element may be required for its expression or an inhibitory cis-element may be contained in the construct.
The minimal cis-regulatory region that drives AC expression is 155bp (mk146-144; 2367-2522). This construct displays variable expression, but the 232-bp construct mk96-144 (2290-2522) expresses in all animals observed.