DAF-2::mNeonGreen was detected in neurons, XXX cells, vulval cells, germ cells, and oocytes. In the last three types of cells, DAF-2::mNeonGreen had clear plasma membrane (PM) localization as expected for a cell surface receptor. In the neurons and XXX cells, a pair of specialized hypodermal cells of neural endocrine function, strong DAF-2::mNeonGreen signals were seen in the cell bodies and along processes. The DAF-2::mNeonGreen-expressing neurons included all of the ciliated sensory neurons marked by the
osm-6 promoter-driven mCherry protein.