In mosaic animals, GFP was detected in almost all tissues, including neurons and muscle cells. Transgenes driving GFP expression under the control of the 263-bp promoter sequence used for
emc-6 rescue experiments induced lethality and did not transmit GFP expression after one generation. To bypass this technical issue, the authors characterized the expression pattern of the Caenorhabditis briggsae ortholog of
emc-6 (
Cb-emc-6) in C. elegans. Interestingly, transgenic expression of Cb-emc- 6 was nontoxic in C. elegans. GFP expression was observed in every tissue from the threefold stage to adulthood. Altogether, these results suggest that
emc-6 is ubiquitously expressed.