Expr3207
Both the K49 and SI85 antibodies, and both arrays, showed that EPS-8 was expressed throughout development, from embryogenesis to adulthood. Expression in post-embryonic stages was prominent in the myoepithelial cells of the pharynx and in the intestine, where EPS-8 localized in the brush border, as it was more apical than the intermediate filaments of the terminal web.
Expr14600
Consistent with the report of Heinick et al., we found that CATP-5::GFP is expressed in the intestinal cells, where it localizes to the apical brush border, and the excretory cell, where it appears to be distributed throughout the cytosol. In addition, we observed CATP-5::GFP expression in the spermatheca (localized to the apical face) and amphid sensory neurons (localized to the sensilla).
Expr2913
MH33 reactivity in the adult worm is completely restricted to the central region of the intestine, apparently surrounding the intestinal lumen. This is seen more clearly with the isolated adult intestine: MH33 reacts with a tubular structure that can often be seen protruding from the cut end of a dissected intestine where the gut cell cytoplasm has apparently dissociated. This structure presumably corresponds to the ``endotube'' described in the intestines of many larger nematodes. Immunoelectron microscopy shows that MH33 reacts exclusively with a discrete circumferential layer within the terminal web, closely underlying the intestinal microvillar brush border. The MH33 reactive layer continues directly into the electron dense regions that define the CeAJ and that join adjacent gut cells.