Using semi-quantitative RT-PCR, we show that C54G7.3 is somatically expressed. C54G7.3 is first detected at 24 h following hatching, when grown at 25 C. C54G7.3 continued to be expressed at 48 h and 72 h. Adults first appeared at the 72 h time point and C54G7.3 showed clear expression despite the significant attenuation of the germline.C54G7.3 and chs-2
expression declined following onset of adulthood (72 h). C54G7.3 was expressed in the absence of a germline and the expression declined once all the worms reached adulthood. Only one of the two predicted transcripts of C54G7.3 includes sequence coding for the PDA domain.