In the posterior pharyngeal bulb gfp::
hum-7 is expressed throughout, including the
pm6 and
pm7 cells that help the grinder contract during feeding (Worm Atlas), while
hmr-1::mKate2 is enriched only in apical regions, as expected.
hmr-1::mKate2 is expressed all through the nerve ring axons, while gfp::
hum-7 is expressed in adjacent cells, perhaps glia. Viewing this same strain on the surface shows that while hmr- 1::mKate2 is highly expressed in the seam cells, gfp::
hum-7 shows no obvious overlap in the seam cells. A strain expressing RFP under control of the myosin heavy chain promoter, Pmyo-3::rfp (Viveiros et al., 2011), shares expression with gfp::
hum-7 in body wall muscles. Therefore gfp::
hum-7 appears to have broad expression, with enrichment in several types of muscle tissue including regions of the pharynx, and in body wall muscles. Expression is not overall enhanced in neurons, but may include support cells for neurons. Expression does not appear enhanced in epidermal cells, although there is precedent for epidermal signal to appear striped due to impingement from muscle or pharynx (Worm Atlas). Antibody staining with antibodies specific to body wall muscle support that at least some of the striped signals are in muscle.