KLP-20 expression was first observed during early epidermal intercalation of the embryo in neuronal precursor cells and was expressed in a similar fashion throughout epiboly. In comma and post-comma stage embryos, KLP-20 is expressed in the developing nervous system, with the bulk of its expression seen in the anterior region of the embryo and with some expression at the posterior-most region, which will go on to form the tail ganglia. In early larval-stage worms, we observed KLP-20 localization in the nerve ring, anterior and posterior ganglia, dorsal and ventral nerve cords, as well as the mechanosensory neurons PLM and ALM. We observed that there is strong colocalization between DiI localization in the amphid neurons and
klp-20::GFP, but
klp-20 expression is not limited to the amphid neurons. KLP-20 was not observed to be expressed in the epidermis.