The
hnd-1 reporters expressed GFP in the MS, C and D embryonic lineages. Expression was first observed in four MS great-granddaughters, four C great-granddaughters and two D daughters. Expression continued through one cell division and then became difficult to detect using
hnd-1(FL)::GFP.
hnd-1(N)::GFP remained detectable in some cells within these MS and C lineages, but disappeared from most body muscle cells by the comma stage of embryogenesis. Then, the
hnd-1 reporters were expressed in Z1 and Z4 as they approached the primordial germ cells to form the gonadal primordium. Shortly after the primordium was assembled,
hnd-1(N)::GFP expression was reduced or disappeared. GFP was not detected in the somatic and germline precursors at hatching or post-embryonically. Therefore,
hnd-1 appears to be expressed during embryogenesis in mesodermal precursor cells that generate predominantly body wall muscle, and then in somatic gonadal precursor cells.