This construct was expressed faintly in QL and QR, more strongly in the neighboring epidermal cells (dorsal hyp7 cells, ventral P cells and lateral V cells), and in dorsal and ventral body muscle cells.
Staining was observed in all larval and adult stages, localizing to the nuclei of the hypodermal cells, specifically the head (hyp3, hyp4, hyp6, and hyp7) and seam/body (hyp7, H, V, and P) hypodermal cells.
In wild-type males, REF-1::GFP can be detected in V6- and T-derived seam cells during the L1 and L2 larval stages. After L2, however, REF-1::GFP is undetectable in the V ray lineage.