To determine where
lite-1 is expressed and identify likely sites of
lite-1 function, the authors generated and examined worms carrying one of four transgenes derived from the wild-type
lite-1 locus: a genomic fragment (
lite-1 genomic), a transcriptional fusion (
lite-1prom::gfp), a C-terminal translational fusion (
lite-1prom::
lite-1::gfp), and an N-terminal translational fusion (
lite-1prom::gfp::
lite-1). GFP expression was observed in a total of 29 cells: pharyngeal neurons M1, M4, M5, and MI; non-pharyngeal neurons ASK, ADL, ASI, ASH, AVG, AVB, RIM, ADF, PHA, PHB, and PVT; and non-neuronal cells Hyp3 (hypoderm), AMso (amphid socket cells), and PHso (phasmid socket cells). AVB was observed only with the C-terminal fusion transgene, and RIM and ADF were observed only with the transcriptional fusion transgene.
lite-1 expression in AVG and PVT was previously reported.