Northern blot analysis showed that the cid-1/cde-1 signal was drastically decreased in the glp-4(bn2) mutant, which lacks a germline compared to wild-type worms, confirming its germline localization.
On whole mounts, clear staining of the gonad arms was visible and no other expression patterns were detected. Closer inspection on extruded gonads showed cid-1/cde-1 mRNA throughout the entire gonad, without clear localization to a specific region.