GFP::WSP-1 was found in punctate structures at the anchor cells (AC) invasive cell membrane that colocalized with F-actin puncta, indicating that WSP-1 localizes to AC invadopodia.
In the somatic gonad, GFP::HLH-2 was expressed at low levels in the Z1/Z4 somatic gonadal precursor cells at the L1 stage. During the time of the AC/VU decision, GFP::HLH-2 was expressed in both pre-AC and pre-VU cells, and after the AC/VU decision, GFP::HLH-2 was reduced in the VU and its descendants. GFP::HLH-2 persisted in the AC through the time of invasion.