Double-antibody staining against SYD-2 and UNC-10 revealed that SYD-2 colocalizes with UNC-10 as discrete puncta of ~200-400 nm in diameter along the dorsal, ventral, and lateral nerve cords. Double-antibody staining against SYD-2 and UNC-13 revealed that SYD-2 also colocalizes with UNC-13. In both cases, the degree of colocalization was very high but not complete [92.9 +/- 2.3% for UNC-10 (n = 5) and 83.1 +/- 6.2% for UNC-13 (n = 6)]. Some SYD-2 puncta appear to stain with varying amounts of UNC-10 or UNC-13 and vice versa, and in some cases, SYD-2 puncta do not costain at all with UNC-10 or UNC-13.
No GFP expression was seen. The gene model transcript tagged with this fusion, syd-9a, might not be real: the translational reading frame in the first exon of syd-9a is shifted as compared to the other syd-9 transcripts.
The GFP expression pattern looks like that obtained from fUL#JW135, with gfp inserted before the syd-9c stop codon but without disruption of expression arising from syd-9d, although the expression may be somewhat weaker.
The GFP expression pattern looks like that driven by fUL#JW135, with gfp inserted before the syd-9c stop codon, but weaker. However, embryonic GFP expression was stronger than in other stages.