When fused to a GFP reporter, the 21-kb region upstream of
nsy-7 drove expression in numerous cell types including gut, the amphid sheath glial cells, and head and tail neurons including AWC, ASE, and ASH. In adult animals,
nsy-7::GFP was asymmetrically expressed in one AWC neuron. The
nsy-7-expressing neuron was identified by crossing a line expressing
nsy-7::GFP with a line expressing an
srsx-3::mCherry (AWCOFF) reporter or a
str-2::dsRed2 (AWCON) reporter.
nsy-7 was consistently expressed in the same cell as
str-2 (145/158 animals), and contralateral to the cell expressing
srsx-3 (99/99 animals). Therefore,
nsy-7 appears to be expressed in AWCON but not AWCOFF.