To identify
npr-2-expressing cells, we generated transgenic animals expressing GFP under control of the rescuing
npr-2 promoter and identified a total of 15-17 GFP-positive neurons that likely express
npr-2. These neurons include sensory neurons OLQs (2-4), ASHL/R, ADFL/R, FLPL/R, and PVDL/R and interneurons AIZL/R, SABD, and PVQL/R. The expressivity of the
npr-2p::GFP transgene in each class of neurons ranges from 62.5% for the AIZ interneurons and 100% for the FLP sensory neurons. The variable penetrance of GFP expression in these neurons might be caused by transgene mosaicism or variable GFP expression intensity.