spe-6 mRNA could be amplified from
fem-3(
q23gf) hermaphrodite mRNA (this mutant has hermphrodite soma but makes only sperm germline), but not not from
fem-1(
hc17ts) hermaphrodite mRNA (mutant hermaphrodites make only oocytes, no sperm); cDNA clone
ca01c03 (GenBank accesion nos. AW057333 and AW057136) are from spermatogenesis-specific libraries; SPE-6 protein only detected in extracts from sperm-producing worms (i.e.
fem-3(gf), not
fem-1(lf) on western blots.