A fosmid reporter wgIs54[
egl-5::EGFP] was expressed in PDA, PDB, DVB, PHBL/R, LUAL/R, PVCL/R, and PLML/R neurons. Among the MNs,
egl-5 was expressed in the most posterior cell of the DA, VA, DD, and VD types of MNs, namely DA9, VA12, DD6, and VD13. Among them, DA9, VA12, and VD13 did not express either
mab-5 or
lin-39, while DD6 was the only neuron in the nervous system that expressed three Hox genes,
mab-5,
lin-39, and
egl-5. Outside of the tail region,
egl-5 was also expressed in the terminally differentiated HSN neurons around the vulva.