::gfp] transcriptional and translational fusions. In case of trxr-2
transcriptional constructs, both 1.5 kb external (EX) promoter of the operon containing trxr-2
and 0.3 kb internal (IN) promoter were amplified separately using ZK632 as a template, and then subcloned into pPD95.77 at SalI/BamHI, and HindIII/SalI, respectively. To generate a translational pEX::trxr-2
::gfp, 2.9 kb full length genomic sequence was amplified and subcloned into the pEX::gfp at SalI and BamHI. To generate a translational pIN::trxr-2
::gfp, the 2.9 kb full length genomic sequence with the 0.3 kb internal promoter was amplified using cosmid ZK637 as a template, and then, the amplified region was subcloned into BamHI and SalI sites of pPD95.77.