A 5-kb genomic fragment containing the first three exons of pad-2
and 3 kb of upstream sequence (the closest upstream gene, R01H10.8, is located 2 kb from pad-2
) was cloned into pPD95.69. This construct, called pGEN3, expresses the PAD-2::GFP fusion protein tagged with a nuclear localization signal under the control of the pad-2
endogenous promoter. --precise ends.