pCZ843

AC3259 (5'-tgatgtccatggggcaggcaagccgcc-3') and AC3260 (5'-ggcggcttgcctgccccatggacatca-3') for Ser86AlaAC3272 (5'-gcagacatcgaagtcaatggagccggctaccca-3') and AC3273(5'-tgggtagccggctccattgacttcgatgtctgc-3') for Ser71Ala

pNU792

612bp eft-3 promoter, 4303bp Cas9 including 3 synthetic introns, NLS and HA-tag, 252bp tbb-2 3'UTR

pAD76

1-3 kb of sod-3 upstream DNA was used to generated this construct.

pTY1544

Clone = pTY1544 | GFP is inserted between amino acids 3-4 of SDC-3.

JK2421

Heat shock promoter fused with lacZ and wild type fem-3 3' UTR.

pAY37

To generate a kcc-3::gfp translational fusion (pAY37), we inserted a gfp-coding sequence into the 3' end of the kcc-3-coding sequence flanked by the 3.9 kb 5' upstream and the 2.2 kb 3' downstream genomic sequence.

pJB11

[nlp-3::GFP] transcriptional fusion. pJB11 has a ~4 kb nlp-3 promoter region inserted into multiple cloning site I (MCS I) of pPD49.26, followed by the GFP coding sequence inserted into MCSII, and by ~750 bp of the nlp-3 3' untranslated region inserted into MCSIII.

pTY656

Clone = pTY656 | provides excess SDC-3.

pAY33

We fused the 0.3 kb promoter sequences of ptr-10 to a kcc-3 cDNA attached with the unc-54 3'UTR sequence. A kcc-3 cDNA clone (yk611h3) was provided by Yuji Kohara.

pBK143

Mutated elo-2 3'UTR. To generate this transgene, the Quikchange II kit was used to delete the predicted miR-786 binding site from the elo-2 3 UTR from pBK134 (the elo-2prom::gfp::elo-2 3'UTR reporter). For this Quikchange reaction, the following primers were used: (5'-gtcttgtttttctgactcatcattttttcaactttagattaatttctttgttaaatttatttttcattg-3' and 5'-caatgaaaaataaatttaacaaagaaattaatctaaagttgaaaaaatgatgagtcagaaaaacaagac-3').