::GFP reporter pCR2 was made by inserting a 366 bp XbaI/SacI(filled) fragment containing the 247 bp minimal vit-2
promoter from pFPO-gfp-V3(FUB1) (gift of M. MacMorris and T. Blumenthal) into XbaI/SmaI cut pPD95.70 (gift of A. Fire). The resulting plasmid has the minimal 247 bp vit-2
promoter followed by the first 14 amino acids of vit-2
, a synthetic intron and the GFP coding sequence.