(FL)::gfp, contains 6.7 kb of upstream sequences, followed by genomic sequences containing the complete fkh-6
coding region, and the gfp-coding region fused in frame immediately 5 to the FKH-6 stop codon. This reporter fully rescued fkh-6
male gonadal defects and restored hermaphrodite self-fertility, suggesting appropriate spatial and temporal expression. The fkh-6
(FL)::gfp (pWC2) rescuing GFP was made by inserting a genomic DNA PCR fragment, from 6708 upstream of the predicted start codon to 3402 downstream, into pPD95.67. --precise ends.