Clone = pJW33. To make the myo-2
-gfp fusion (pJW33), a PCR fragment containing gfp coding sequences and an engineered stop codon was inserted in frame at the 3' end of the egl-20
cDNA. The gfp coding sequence was amplified using the primer pair JW30 (5'-CCGCTGCAGATGAGTAAAGGA GAAGAACTT-3') and JW31 (5'-CCGGAGCTCTTATTTGTATAGTTCATCCAT-3'. The gfp-tagged egl-20
cDNA was then inserted into NheI-SacI digested pPD30.69, a plasmid containing myo-2
promoter sequences (from A. Fire) (Fire et al., 1990). The extrachromosomal array muEx68 was generated by injecting pJW33 at 20 ng/ul (Whangbo and Kenyon, 1999). | Conflicting genotype: [myo-2
antisense)] in Whangbo et al., 2000.