mec-4 promoter driving tetR-L-QFAD in an RCME vector. The mec-4 promoter from NMp3735, tetR-L-QF from NMp3821 and the act-4 3'UTR from NMp3751 were co-assembled in a SapI GGreaction.
Pglr-1::CMK-1 was made by PCR of CMK- from Pttx-1::CMK-1::GFP (Gift from Piali Sengupta) adding a 3'stop codon and cloning into pV6 with Nhe1 and Kpn1. pBM13 was injected at 25 ng/μl with Pmyo2::mCherry at 10 ng/μl to make pzEx334.