pSL779

N2 animals were injected with pSL779 at 40 ng/L + 100 ng/L of odr1::rfp to make strain UD522 [ycEx249].

pLC681

efn-4 genomic, kind gift of L. Chen

pGO120

mEos a gift from Loren L Looger. pPD95.75 was used as a backbone.

pC1

pCI is derived from pBX by removing a 1.5 kb BamHI/ClaI fragment upstream of the pha-l gene.

pLJ8

This L-AChR(gf) construct was generated by PCR-based site directed mutagenesis using mutant primers with the wild type cDNA constructs as templates.

pLJ9

This L-AChR(gf) construct was generated by PCR-based site directed mutagenesis using mutant primers with the wild type cDNA constructs as templates.

pMF1

This L-AChR(gf) construct was generated by PCR-based site directed mutagenesis using mutant primers with the wild type cDNA constructs as templates.

Cbace-1

Clone = Cbace-1. A 5.9 kb fragment containing the entire C. briggsae ace-1 gene (2560 bp (2.6 kb) of 50 region upstream of the ATG, 3 kb of genomic coding region and 0.3 kb of 30 sequence downstream of the stop codon) was amplified by PCR using Expand High Fidelity Taq polymerase (Boehringer Mannheim) on l phage jcl#18 (Grauso et al., 1996).

pCXT51

This construct contains multiple copies of the WT SMAD box (SMAD binding site GTCT) organized in a RLR (R, rightward; L, leftward) orientation upstream of the minimal pes-10 promoter and gfp of the Fire Lab Vector L3135, to test whether the expression of the gfp reporter is responsive to Sma/Mab signaling. RAD- SMAD stands for reporter acting downstream of SMAD (Tian et al., 2010).

pHA#380 and pHA#381

Clone = pHA#380 and pHA#381. glr-1p::lin-12(RNAi) animals were generated by co-injection at 40ng/l of plasmids pHA#380 and pHA#381. Both plasmids contain a 5.5kb NotI fragment of lin-12 cDNA from pDS27.4 behind the glr-1 promoter; in pHA#380 and pHA#381 the fragments are in the sense and antisense orientation, respectively. Introduction of pHA#380 alone results in lin-12 gain of function behavioral defects not reported here