PSAB1230

This work (10 ng/l)

pSL779

N2 animals were injected with pSL779 at 40 ng/L + 100 ng/L of odr1::rfp to make strain UD522 [ycEx249].

pLC681

efn-4 genomic, kind gift of L. Chen

pGO120

mEos a gift from Loren L Looger. pPD95.75 was used as a backbone.

NMp3823

mec-4 promoter driving tetR-L-QFAD in an RCME vector. The mec-4 promoter from NMp3735, tetR-L-QF from NMp3821 and the act-4 3'UTR from NMp3751 were co-assembled in a SapI GGreaction.

pC1

pCI is derived from pBX by removing a 1.5 kb BamHI/ClaI fragment upstream of the pha-l gene.

pBM13

Pglr-1::CMK-1 was made by PCR of CMK- from Pttx-1::CMK-1::GFP (Gift from Piali Sengupta) adding a 3'stop codon and cloning into pV6 with Nhe1 and Kpn1. pBM13 was injected at 25 ng/μl with Pmyo2::mCherry at 10 ng/μl to make pzEx334.

pLJ9

This L-AChR(gf) construct was generated by PCR-based site directed mutagenesis using mutant primers with the wild type cDNA constructs as templates.

pLJ8

This L-AChR(gf) construct was generated by PCR-based site directed mutagenesis using mutant primers with the wild type cDNA constructs as templates.

pMF1

This L-AChR(gf) construct was generated by PCR-based site directed mutagenesis using mutant primers with the wild type cDNA constructs as templates.