Clone = pVB518OB. | Using an oligo dT primed first strand cDNA reaction from total C.elegans RNA, the full length tomm-40
cDNA was amplified using primers with flanking AgeI sites. The amplified cDNA, which was designed to lack the stop codon, was inserted between the tomm-40
promoter and the gfp coding region in pVB488OB at the AgeI site to yield pVB518OB which contains a full length tomm-40
::gfp fusion construct.