::gfp] translational fusion. A translational GFP fusion construct was obtained by PCR amplification of the trx-1
promoter and gene from C. elegans genomic DNA. The forward primer B0228.5-fGFP 5$(B!l(B-CATTCTGCAGAGAATGGATACCTGATCATT-3 and the reverse primer B0228.5-rGFP 5$(B!l(B-TCGAGGATCCTTGAGCAGATATCGTGCTCCA-3$(B!l(B were used and the PCR product was cloned into the PstI/BamHI sites of the pPD95.77 vector. MT1642 lin-15
ts) X animals were microinjected with the trx-1
::gfp fusion construct together with the marker plasmid pBHL98 that rescues the multi-vulva phenotype of the MT1642 strain. --precise ends.