Expresses a constitutively active version of let-60
cDNA under the control of a heat shock promoter. The wild-type LET-60 cDNA was obtained from yk1438c09
(a gift of Yuji Kohara). The sequence was modified using a Quickchange kit (Agilent technologies) to create the G12V mutation and subcloned into the pPD49_78 heat shock vector (a gift of A. Fire Stanford University CA) (pRJM137).