::gfp] transcriptional fusion. A 2.1-kb promoter fragment of zmp-2
was amplified from genomic DNA of C. elegans N2 strain using a nested PCR with the 5 PRIME MasterMix (5 PRIME GmbH, Hamburg, Germany). Primers Neu-CeleMMP5-For1 (5-AAGCTTTGGACAAGCAACGCTGAG-3') and MMP5-rev3
(5-AAGTGTGAATAGGGATAAGATAAGGTAGAT-3') were used in the first PCR reaction and nested primers Neu- CeleMMP5-For2 (5-AAGCTTGGTGCATCGATGAAGCTGAA-3') and MMP5Pro-Rev2 (5-CATTATATACCGGATCCAGTCCTCTT-3') were used in a subsequent secondary PCR reaction. The resulting PCR amplification product was cloned by the use of the TA cloning Kit pGEM-T easy (Promega, Mannheim, Germany).The GFP encoding sequence from pPD95 75 (Addgene, Cambridge) was further cloned into the resulting plasmid with the use of BamHI und ApaI restriction endonucleases, according to standard protocols. The final construct included the zmp-2
(2.1)::gfp fusion in the pGEM-T easy backbone and the sequence was verified by custom-sequencing with Sp6 and T7 sequencing primers (GATC Biotech AG, Konstanz, Germany).