The sequences inserted by recombineering consist of C. elegans codon-optimized GFP containing FRT flanked GalK, derived from vector pBALU1 (Tursun et al., 2009), to which we added an Avi-tag (de Boer et al., 2003; Schatz, 1993) for future purification efforts. The GFP-Avi tag was inserted at the 3' ends of the predicted genes. For par-3
we used primers par-3
-F (5'GCCAATACCGTCGCAGAGATCAGGGACCGCCTCATCGTTTTCCCCAGTACGGAGGAGGATCTGGAGGAGGAGGATCTGGAGGAGGA-3') and par-3
-R (5'-GATTCCGTATTTTTCGCGGCTGCGTAATATAACTTTGAGAAAAAACTGACCTATTCATGCCATTCAATCTTCTGAGCTTCG-3'). PCR amplifications were done with KOD hot start polymerase (Manufactured by Toyobo and distributed by Merck Millipore, Billerica, MA, USA) according to manufacturer's instructions, and an annealing temperature of 70 deg C.