translational fusion. GFP was inserted in-frame within the EXP-1 open reading frame. The GFP coding region from pPD95.75 was amplified with primers tagged with ApaI sites on both the 5 and 3 ends. This PCR product was gel-purified and inserted in frame into an ApaI site of pAB03 to generate pAB05. This fusion protein contains GFP in the intracellular loop between M3 and M4. This translational exp-1
:GFP reporter was coinjected at 1 ng/l with pEK1 at 30 ng/l and 69 ng/l of 1-kb DNA Ladder (Gibco) into exp-1
ts) animals. The stable transgenic lines (oxEx469) were rescued for enteric muscle contractions and showed GFP fluorescence in the enteric muscles.