was made by amplifying, via polymerase chain reaction, pop-1
cDNA from pMM414 (Maduro et al. 2002), fusing it with gfp, and inserting it into pMS155 to make pTA1. hlh-2prox
promoter is specific to Z1.pp, Z4.aa and their daughters, the alpha and beta cells (Sallee and Greenwald 2015). In the L2 stage, hlh-2prox
drives expression of GFP in all four cells; initially, before specification, the level of expression is similar in the unspecified alpha and beta cells but later becomes relatively greater in the AC and alpha-VU than in the beta-VUs (Sallee and Greenwald 2015).