was amplified from TOPO cloned arf-6
cDNA using primers B1arf-6B2-F [ggggACAAGTTTGTACAAAAAAGCAGGCTatgggtaaattcctgtcgaaaatct] and arf-6gfpR
[tgaaaagttcttctcctttactcatcggcttgcaattctggcttagccac], and gfp was amplified from Pmab-5::cnt-2
::gfp (Singhvi et al, 2011) using primers arf-6gfp
-F[gtggctaagccagaattgcaagccgATGAGTAAAGGAGAAGAACTTTTCA] and B1gfpB2-R, then the two fragments were PCR-fused and the arf-6
::gfp cDNA was amplified using B1arf-6B2-F and B1gfpB2-R, and finally recombined into pDONR 221.