09318202437763223 E07

A gfp-tagged aptf-1 fosmid. The fosmid wTRG5_09318202437763223_E07_K06A1-1.1_WRM0640B_F12-- S0001_pR6K_Amp_2xTY1ce_EGFP_FRT_rpsl_neo_FRT_3xFlagd[29470..30845]--unc-119- Nat containing gfp-tagged aptf-1 was a gift from M. Sarov.

pMR732

The sur-5 ubiquitous promoter was PCR-amplified from pTG96 (gift from M. Han) using the following primers: 5'-ATATCTGCAGTGCTAAATTGCAACCCCGTG-3' and 5'-AAGTGCGGCCGCCATTCTGAAAACAAAATGTAAAG-3', digested with PstI-NotI and inserted at the corresponding sites into pMR725 to generate sur-5p::AAK-2 (pMR732).

pCR2

The vit-2::GFP reporter pCR2 was made by inserting a 366 bp XbaI/SacI(filled) fragment containing the 247 bp minimal vit-2 promoter from pFPO-gfp-V3'(FUB1) (gift of M. MacMorris and T. Blumenthal) into XbaI/SmaI cut pPD95.70 (gift of A. Fire). The resulting plasmid has the minimal 247 bp vit-2 promoter followed by the first 14 amino acids of vit-2, a synthetic intron and the GFP coding sequence.

WBCnstr00020658

(gift from M. Chalfie)

WBCnstr00015564

M. musculus parvalbumin cDNA was subcloned from pCMV6-parvalbumin (Origene).

WBCnstr00015555

M. musculus parvalbumin cDNA was subcloned from pCMV6-parvalbumin (Origene).

WBCnstr00015556

M. musculus parvalbumin cDNA was subcloned from pCMV6-parvalbumin (Origene).

WBCnstr00015563

M. musculus parvalbumin cDNA was subcloned from pCMV6-parvalbumin (Origene).

WBCnstr00015562

M. musculus parvalbumin cDNA was subcloned from pCMV6-parvalbumin (Origene).

WBCnstr00016677

Construct made by M. Vidal lab; candidate unc-37 target gene.