To construct intestine specific cav-2
clones, we used Gateway multisite technology (Invitrogen, Paisley, UK). Individual entry clones containing the vha-6
promoter and cav-2
cDNA were produced and then combined in a Gateway reaction into the destination vector pHP2 (H. Peterkin and H. Baylis, unpublished data). The vha-6
promoter clone contained a fragment, similar to that previously used by Grant and colleagues (Chen et al., 2006), amplified using the oligonucleotides ggggacaagtttgtacaaaaaagcaggctcgcgttcaccactcgaccaccgaac and ggggacaacttttgtatacaaagttgttttttatgggttttggtaggttttag. The cav-2
cDNA was amplified using the oligonucleotides ggggacaacttttctatacaaagttgaaaaatgactcgtcagaatacttccgaaag and ggggacaactttattatacaaagttgttaaacatgatgaatgtgtttttc.