::gfp]. The gar-3
::GFP expression construct was generated by overlap-extension PCR using wild-type genomic DNA and vector pPD95.77 as templates. The 5' forward primer is located ~6000 bp upstream of the gar-3
ATG. Primers used were: gar-3Pro, 5'-ggt aca cag ctc ctt tcc aat caa-3
, 5'-cag aca cac tta ttc cag atg ttg gcc aaa gga ccc aaa g-3'; gfp-gar-3R
, 5'-ctt tgg gtc ctt tgg cca aca tct gga ata agt gtg tct g-3'; and gfp-R, 5'-caa acc caa acc ttc ttc cga tc-3'. --precise ends.