pGO120

mEos a gift from Loren L Looger. pPD95.75 was used as a backbone.

pGO38

Clone = pGO38. T02B11.3pro a gift from Maya Tevlin. pPD95.75 was used as a backbone.

pPD93_97

(from A. Fire)

pGO93

pha-4pro a gift from Maxwell G. Heiman. pPD95.75 was used as a backbone.

pGO32

Clone = pGO32. vap-1pro a gift from Leo Liu. pPD95.75 was used as a backbone.

pRJM137

Expresses a constitutively active version of let-60 cDNA under the control of a heat shock promoter. The wild-type LET-60 cDNA was obtained from yk1438c09 (a gift of Yuji Kohara). The sequence was modified using a Quickchange kit (Agilent technologies) to create the G12V mutation and subcloned into the pPD49_78 heat shock vector (a gift of A. Fire Stanford University CA) (pRJM137).

pTX(md130)

Clone = pTX(md130) | This construct expresses a truncated syntaxin(1-227) with a few novel aa.

pGO8

lit-1 cDNA (yk1457b04) a gift from Yuji Kohara (AgeI/EcoRI). pPD95.75 was used as a backbone.

pMH86

A plasmid containing rescuing wild-type dpy-20 DNA. pMH86 is a Bluescript (SK+)-derived plasmid that has a 6-kb Xbal DNA insert containing the entire dpy-20 gene subcloned from a dpy-20-containing cosmid; D . Clark and D . Baillie, pers.comm.

pCMH1229

The PEST domain was amplified from pAF207 (a generous gift from Dr. A. Frand, UCLA) and was used to create NLS-GFP- pest::unc-54-3'UTR (pCMH1225). A a 2.6kb fragment (chr I:6,170,232..6,178,361) was subcloned into pCMH1225 using XmaI sites.