Clone = pBL174. The egl-2
cDNA splice isoform in this paper differs from the other reported isoform in two ways (Weinshenker et al., 1999): 3 amino acids, IKV, are added between TSSNFNQV and MNLGGDML, and 8 amino acids, LRPFYLIS, are removed from between FNIAFKNS and SRENPGGG. Further, for this construct, the egl-2
gf) point mutation was introduced via single site mutagenesis.