::gfp] translational fusion. The kgb-1
::gfp plasmid was made by fusion of the GFP coding sequence to a 1.770-kbp genomic fragment upstream of the kgb-1
translational start codon (Pkgb-1) and the KGB-1 coding sequence without the last two N-terminal codons. The cDNA of kgb-1
was amplified by PCR (primers: 5'AGTGGATCCGCCGGTACATAACGA-3', 5'-TTGGTACCCCAGTGAAAATGTCGTGGTCGG-3') and cloned into pJET1/blunt (GeneJETTM PCR Cloning Kit, Fermentas, St. Leon-Rot, Germany). BamHI/KpnI digestion enabled cloning of the kgb-1
fragment (1.157 kbp) into the Pkgb-1::gfp plasmid (between Pkgb-1 and gfp without NLS signal).