To generate the Pkri-1::gfp::kri-1
(pJR32) construct, an AscI/BamHI mec-7
promoter fragment was removed from the L3691/pPD117.01 N-terminal GFP vector to generate plasmid pJR7. A full length kri-1a
cDNA (2190 bp) fragment (GenBank accession number DQ372920) was inserted downstream of and in frame with gfp at the NheI site of pJR7, and 1.5 kb of kri-1
5$(B!l(B regulatory sequence was inserted at the ClaI and XmaI sites. All critical regions were fully sequenced, including the entire cDNA. --precise ends.