-tagRFP minigene composed of 4,070 bp of unc-49
promoter, a genomic DNA fragment containing the first four exons of unc-49
, a cDNA fragment corresponding to the last eight exons of unc-49B
isoform and the 3' UTR sequence of unc-54
. The tagRFP sequence was inserted in the TM3TM4 intracellular loop of the UNC-49B subunit. The unc-49B
-tagRFP minigene fused with the 3' UTR sequence of unc-54
was inserted into pCFJ151 by using SbfI and SpeI sites.