8481741786659566 C06

WBCnstr00020352

The mtf-1/sun-1-coding locus including 2.8 kb of its 5' sequence and 780 bp of its 3' regulatory sequence was inserted into the pEGFP-1 vector of BD Biosciences Clontech (#6086-1).

WBCnstr00020276

Created by cloning the sun-1 coding sequence, together with 468 and 780 bp upstream and downstream, respectively. Codon-optimized mRuby (including a single intron from the zyg-12 gene), together with a 3x(Gly-Gly-Ser-Gly) linker, was fused to the SUN-1 C-terminus.

WBCnstr00023067

Construct was generated using Gibson assembly (Gibson et al., 2009), and verified by sequencing of new junction regions. Psun-1::TIR1::mRuby::sun-1 3'UTR was derived from pCFJ178. A codon-optimized TIR1 gene with two introns was assembled from multiple gBlocks (IDT). This coding sequence was used to replace the sun-1 coding sequence in pOR131 (Rog and Dernburg, 2015), a derivative of pCFJ178 containing the sun-1 promoter and coding sequence inserted upstream of a codon-optimized mRuby gene, followed by the sun-1 3'UTR. In the resulting construct, TIR1 and mRuby are separated by a 3x(Gly-Gly-Ser-Gly) linker.

pZZ884

chc-1(genomic chimera)::gfp under the control of the ced-1 promoter (Pced-1).

pQS41

epn-1(genomic DNA)::gfp under Pepn-1 control.

pKO114

the zyg-1 promoter has been mutated for the three EFL-1-DPL-1 binding sites in this construct

pCZGY534

Clone = pCZGY534 | Injected into cebp-1(ju659);rpm-1;muIs32 animals.

pCZGY536

Clone = pCZGY536 | Injected into cebp-1(ju659);rpm-1;muIs32 animals.

pCZGY757

Clone = pCZGY757 | Injected into cebp-1(ju659); rpm-1; juIs1 animals.