] translational fusion. A genomic fragment spanning the ORF in mbk-2c
was cloned into two vectors, pJH4.52 and pID3.01, to create amino-terminal GFP fusions. These vectors utilize pie-1
5' and 3' UTR sequences to drive expression of the transgene in the maternal germline. GFP lines were created by the complex array method or by the microparticle bombardment method (pID3.01). Several independent lines were established using each technique. Although expression levels varied from line to line, all lines showed the same overall distribution of GFP:MBK-2.