Mouse monoclonal antibodies against EMB-4 were generated by Abmart Inc. We obtained the cell lines for two of these antibodies, clones 7K3-12 (generated against peptide HFEDMDHEMQ, residues 1437-1446 in EMB-4) and 5M19-8 (generated against peptide ETEHEKKHRE, residues 1409-1418 in EMB-4). EMB-4 antibodies were purified from the supernatants from the 7K3-12 and 5M19-8 hybridoma cell lines using the Mouse TSC Purification System from Abcam. The antibody used in this study is the 7K3-12 clone. Clone 5M19-8 was predominantly used in (Akay et al., 2017). Both antibodies were tested for specificity by western blotting and immunofluorescence experiments on wild-type animals and emb-4(hc60) mutants.
emb-4