An anti-peptide antibody was raised in rabbits against a peptide with the sequence NH2-CTLEKKEGDEEKQAS-CONH2, corresponding to the residues 650-663 of C. elegans TRXR-1 protein. trxr-1
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1). csc-1
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1). bub-1