Antibody against NLG-1 was prepared basically as described (Huang et al., 2014). The nlg-1 cDNA fragment encoding C- terminal of NLG-1 was cloned into pGEX5X-1 vector containing GST tag. Polyclonal rabbit antibody against GST-NLG-1 was generated and affinity-purified as described (Perrone et al., 1998).
nlg-1