The region encoding amino acids 338-538 of CSR-1b was amplified from an N2 cDNA library and cloned into pGEX6P-1. GST fusions were purified from bacteria and outsourced for injection into rabbits (Covance, Princeton, NJ). CSR-1 antibodies were affinity purified using a MBP fusion of the same antigen region, as previously described.
csr-1