Anti-W10G11.20/DNC-3 rabbit serum was made against GST- fused N-terminal truncated W10G11.20/DNC-3 (49 to 171). The W10G11.20/DNC-3 fragment was amplified from a cDNA library and cloned into the entry vector pENTR201 (Invitrogen). The entry clone was recombined with pDEST15 (Invitrogen) by LR recombination. The obtained plasmid was used for protein expression in E. coli, and GST-W10G11.20/DNC-3 was purified using GST-sepharose (GE Healthcare, Pollards Wood, UK).
dnc-3