Briefly, for the UNC-38 antibody, a DNA fragment encoding 375-418 amino acids of the UNC-38 gene was cloned in pGEX-5X. The GST::UNC-29 and GST::UNC-38 fusion proteins were then expressed in BL21 E. coli cells and purified. This fusion protein was then injected into the rabbits and rabbits were boosted with 100 μg each time. The antibody was then purified and used for experiments. The antibody production and purification was performed by Bioklone Biotech Pvt. Ltd, Chennai, India.
unc-38