Full-length RPL-5, RPL-25.2, RPS-3 and RPS-0 ribosomal proteins tagged with six Histidine residues (RPL-5-His6, RPL-25.2-His6, RPS-3-His6, RPS-0-His6) were expressed in E. coli. The recombinant proteins were purified by Ni-NTA agarose beads and the eluted RPL-5-His6, RPS-3-His6 and RPS-0-His6 proteins were used to raise polyclonal antibodies in rat. The RPL-5 and RPS-0 polyclonal antibodies were further purified using RPL-5 or RPS-0 recombinant proteins.
rps-3