A protein fragment corresponding to amino acids 124-190 of ALFA-1 fused to glutathione S- transferase (GST) was expressed, purified using glutathione Sepharose 4B (Amersham Biosciences) and used to raise rabbit anti-ALFA-1 antibodies. Antisera were generated by Pocono Rabbit Farm and Laboratory. Specific antibodies were affinity-purified using an identical ALFA-1 protein fragment fused to maltose-binding protein (MBP) and coupled to Affigel 10 (Bio-Rad).
alfa-1